airway epithelial cells  (Cell Applications Inc)

Journal: mBio

Article Title: Outcomes of experimental infection of calves with swine influenza H3N2 virus

doi: 10.1128/mbio.03957-24

Figure Lengend Snippet: Bovine primary respiratory and immortalized mammary gland cells infected with different swine and avian IAVs. (A) Bovine primary nasal turbinate and tracheal epithelial cells, as well as immortalized mammary gland epithelial cells and fibroblasts, were infected with swine H3N2, avian rgH5N1/PR8, or avian H9N2 viruses at a multiplicity of infection (MOI) of 1. At 24 h post-infection, cells were fixed and stained with an anti-IAV NP monoclonal antibody. (B) Mean values of the integrated fluorescence density from three separate biological repeats were compared among bovine cells infected with different IAVs. The result was analyzed by using a one-way ANOVA followed by Tukey’s multiple comparison. (C) Four bovine cells were infected with swine H3N2, avian rgH5N1/PR8, or avian H9N2 viruses at an MOI of 0.1. The supernatants were collected at 12 and 36 h post-infection and were titrated on MDCK cells. The result was analyzed by using a two-way ANOVA (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001).

Article Snippet: Airway epithelial cells were incubated at 37°C with 5% CO 2 and maintained using bronchia/trachea epithelial cell basal medium (Cell Applications, Inc.) supplemented with 10% FBS (Gibco, Waltham, MA, USA).

Techniques: Infection, Staining, Fluorescence, Comparison

Journal: mBio

Article Title: Outcomes of experimental infection of calves with swine influenza H3N2 virus

doi: 10.1128/mbio.03957-24

Figure Lengend Snippet: Bovine primary respiratory and immortalized mammary gland cells infected with PR8 and rgH5N1/PR8. (A) Bovine primary nasal turbinate and tracheal epithelial cells, as well as immortalized mammary gland epithelial cells and fibroblasts, were infected with the PR8 or rgH5N1/PR8 at a multiplicity of infection of 1. At 24 h post-infection, cells were fixed and stained with an anti-IAV NP monoclonal antibody. (B) Mean values of integrated fluorescence density from three separate biological repeats were compared in each bovine cell line infected with PR8 or rgH5N1/PR8. The result was analyzed by using a Student’s t test (** P < 0.01, *** P < 0.001, and **** P < 0.0001).

Article Snippet: Airway epithelial cells were incubated at 37°C with 5% CO 2 and maintained using bronchia/trachea epithelial cell basal medium (Cell Applications, Inc.) supplemented with 10% FBS (Gibco, Waltham, MA, USA).

Techniques: Infection, Staining, Fluorescence